Description

Performs alignment of BS-Seq reads using bismark

Input

name:type
description
pattern

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

reads

:file

List of input FastQ files of size 1 and 2 for single-end and paired-end data, respectively.

meta2

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

fasta

:file

Reference genome FASTA file

meta3

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

index

:directory

Bismark genome index directory

BismarkIndex

Output

name:type
description
pattern

bam

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*bam

:file

Output BAM file containing read alignments

*.{bam}

report

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*report.txt

:file

Bismark alignment reports

*{report.txt}

unmapped

meta

:map

Groovy Map containing sample information e.g. [ id:‘test’, single_end:false ]

*fq.gz

:file

Output FastQ file(s) containing unmapped reads

*.{fq.gz}

versions

versions.yml

:file

File containing software versions

versions.yml

Tools

bismark
GPL-3.0-or-later

Bismark is a tool to map bisulfite treated sequencing reads and perform methylation calling in a quick and easy-to-use fashion.